Nanobio Laboratory (LNB)

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The Nanobio Laboratory (LNB) is part of the Biological Sample Preparation Village* and offers assistance in preparing samples for research focused on exploring the interaction between nanomaterials and biomolecules, as well as for studies requiring contrast enhancement and resin embedding of cells or tissues. The LNB is situated on axis 40, adjacent to the Cateretê beamline.

*The Biological Sample Preparation Village aims to support internal and external users in the preparation of biological samples, as well as in conducting sectioning, syntheses, and characterizations for biological studies.

CONTACT & STAFF

Contact: Flavia Elisa Galdino
Tel.: +55 19 3512 1184
E-mail: flavia.galdino@lnls.br

AVAILABLE INFRASTRUCTURE

INCUBATORS/THERMAL TREATMENT

Eppendorf ThermoMixer® C

Performs sample incubation at controlled temperature (1 to 99 °C) and under agitation (300 – 2000 rpm, depending on the support used). It includes support for agitating samples in 2 mL microtubes, 15 mL conical centrifuge tubes and culture plates (128 × 85 mm).

Access the specifications here: Eppendorf ThermoMixer® C

IKA C-MAG HS 7 Stirrer with Digital Temperature Controller

Performs sample stirring under controlled temperature conditions (from room temperature up to 500 °C), with agitation ranging from 50 to 1500 rpm using a magnetic stirring bar.

Access the specifications here: Descrição – C-MAG HS 7 control

SolidSteel SSD-40L Drying Oven

Performs thermal treatment or drying of samples within a temperature range from 5 °C above room temperature up to 250 °C.

FANEM 1102 Water Bath

Provides heating for liquid or solid substances that cannot be directly exposed to a heat source, operating within a temperature range from 5 °C above room temperature up to 80 °C.

CENTRIFUGES

Eppendorf 5804 Benchtop Centrifuge

Performs the centrifugation of samples in 2 mL microtubes (max. 14,000 rpm) and conical centrifuge tubes of 15 and 50 mL (max. 5000 rpm). It does not have temperature control.

Access the specifications here: Centrifuge 5804 / 5804 R | Rendimento médio, alta versatilidade

Hitachi CR22N High-Speed Centrifuge

Performs the centrifugation of samples with temperature control (-20 to 40 °C) in 50 mL conical centrifuge tubes (from 300 to 18,000 rpm) and in 2 mL microtubes (up to 22,000 rpm).

Access the specifications here: Himac Product Line – Eppendorf

Additional Equipment

Additionally, the LNB is equipped with a fume hood for chemical manipulations, a muffle furnace (EDG 1800), a refrigerator with a freezer (4 °C and -20 °C), an ice machine (Hexicryo), a helical agitator for the preparation of polymeric particles (IKA RW 20.N), ultrasonic baths (Eco-sonic and Branson), a pH meter (Sensoglass), and a stereomicroscope (Olympus SZ61).

GENERAL RULES FOR USING THE LNB INFRASTRUCTURE

On-site training and use of PPE (Personal Protective Equipment).
To access the LNB (NanoBio Laboratory), it is mandatory to wear lab coat (long sleeve), safety glasses and nitrile gloves. In addition to PPE, it is compulsory to wear suitable clothing (long pants and fully closed shoes that cover the back of the foot).
Declare all samples and reagents on the Safety Form according to the submitted proposal.
Chemical reagents and samples not declared on the Safety Form, as per the submitted proposal, will be prohibited. Changes and additions must be formalized in advance and authorized by the Safety Assessment Committee.
Only bring to the experimental facilities (beamlines) the materials necessary to conduct the experiments.
Reagents and samples should be temporarily stored for the duration of the experiments. After this period, the materials must be removed by the respective user.
Materials without proper identification will not be accepted. All samples and chemical reagents must be properly identified. Identification must be legible. Toxic or volatile materials will be stored in the fume hood.
Keep the LNB in working order, ensuring its organization and internal operating rules in accordance with safety guidelines and recommendations.
Only trained users, in person, will be authorized and have access to the LNB.