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Nanobio Laboratory (LNB)
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The Nanobio Laboratory (LNB) is part of the Biological Sample Preparation Village* and offers assistance in preparing samples for research focused on exploring the interaction between nanomaterials and biomolecules. The LNB is situated on axis 40, adjacent to the Cateretê beamline.

This laboratory is equipped with incubators that enable sample incubation under controlled temperature conditions, both in a stationary manner and under agitation. Additionally, the laboratory is equipped with centrifuges and instrumentation for the characterization of these samples, employing techniques such as dynamic light scattering and ultraviolet and infrared spectroscopy.

*The Biological Sample Preparation Village aims to support internal and external users in the preparation of biological samples, as well as in conducting sectioning, syntheses, and characterizations for biological studies.

CONTACT & STAFF

Contact: Flavia Elisa Galdino
Tel.: +55 19 3512 1184
E-mail: flavia.galdino@lnls.br

AVAILABLE INFRASTRUCTURE

INCUBATORS

Eppendorf ThermoMixer® C

Performs sample incubation at controlled temperature (1 to 100 °C) and under agitation (300 – 2000 rpm, depending on the support used). It includes support for agitating samples in 2 mL microtubes and 15 mL conical centrifuge tubes. 

Access the specifications here.

Refrigerated Shaking Incubator TE-424 - Tecnal

Used for incubating samples in large volumes at a controlled temperature (10 to 60 °C) and under orbital shaking (30 – 250 rpm). In the LNB, supports are available for 125 and 250 mL Erlenmeyer flasks. 

Access the specifications here.

CENTRIFUGES

Eppendorf 5804 Benchtop Centrifuge

Performs the centrifugation of samples in 2 mL microtubes (max. 14,000 rpm) and conical centrifuge tubes of 15 and 50 mL (max. 5000 rpm). It does not have temperature control. 

Access the specifications here.

High-Speed Centrifuge Hitachi CR22N

Performs the centrifugation of samples in 50 mL conical centrifuge tubes with temperature control (-20 to 40 °C) and speeds ranging from 300 to 18,000 rpm.

Access the specifications here.

CHARACTERIZATIONS

Dynamic Light Scattering Malvern Nano-ZS

Used for characterizing the hydrodynamic diameter, polydispersity index, and zeta potential of nanoparticles in suspension. It has temperature control and a coupled system that allows for pH control of the solution through acid-base titration. 

Access the specifications here.

UV/Vis Spectrophotometer Agilent 8453

Determines the intensity and absorption bands of samples in the wavelength range of 190 to 1100 nm. 

Access the specifications here.

Infrared Spectrophotometer (FTIR) Spectrum Two

Performs the characterization of samples using FTIR and ATR mode in the wavenumber range of 8300 to 350 cm-1. 

Access the specifications here.

More

Additionally, the LNB is equipped with a fume hood for chemical manipulations, a refrigerator with a freezer (-20°C and 4°C), a helical agitator for the preparation of polymeric particles (IKA RW 20.N), ultrasonic baths (Eco-sonic and Branson), pH meter (W3B), viscometer (AND SV-10), muffle furnace (EDG 1800), sample drying oven (SolidSteel SSD-40L), and a stereomicroscope (Olympus SZ61).

GENERAL RULES FOR USING THE LNB INFRASTRUCTURE

  • On-site training and use of PPE (Personal Protective Equipment).
  • To access the LNB (NanoBio Laboratory), it is mandatory to wear lab coat (long sleeve), safety glasses and nitrile gloves. In addition to PPE, it is compulsory to wear suitable clothing (long pants and fully closed shoes that cover the back of the foot).
  • Declare all samples and reagents on the Safety Form according to the submitted proposal.
  • Chemical reagents and samples not declared on the Safety Form, as per the submitted proposal, will be prohibited. Changes and additions must be formalized in advance and authorized by the Safety Assessment Committee.
  • Only bring to the experimental facilities (beamlines) the materials necessary to conduct the experiments.
  • Reagents and samples should be temporarily stored for the duration of the experiments. After this period, the materials must be removed by the respective user.
  • Materials without proper identification will not be accepted. All samples and chemical reagents must be properly identified. Identification must be legible. Toxic or volatile materials will be stored in the fume hood.
  • Keep the LNB in working order, ensuring its organization and internal operating rules in accordance with safety guidelines and recommendations.
  • Only trained users, in person, will be authorized and have access to the LNB.